FLT4 anticorps (Extracellular Domain) (Biotin)

N° du produit ABIN1589800

Cet anticorps Souris Monoclonal détecte spécifiquement FLT4 dans WB, ELISA et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour FLT4 anticorps (Extracellular Domain) (Biotin) (ABIN1589800)

Antigène: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FLT4 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1(9D9)

Détail du produit anti-FLT4 anticorps (Biotin)

Épitope: Extracellular Domain

Fonction: VEGFR-3/FLT-4 antibody

Specificité: Recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain

Réactivité croisée (Details): The unconjugated antibody will detect native human VEGFR-3/FLT-4 in ELISA experiments.

Attributs du produit: Chromosomal location: 5q35.3
The antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain. VEGFR-3/FLT-4 mediates the angiogenic activity of VEGF-C and VEGF-D on lymphatic endothelial cells.

Purification: The IgG1 fraction of the hybridoma supernatant was purified by Protein G affinity chromatography and then biotinylated using a standard protocol. VEGFR-3/FLT-4, belongs to the class III subfamily of receptor tyrosine kinases.

Immunogène: recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain

Isotype: IgG1

Information d'application

Indications d'application: WB: Use at 2-5 μg/mL, ELISA: Use at 1-10 μg/mL, IP: use at 2-10 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Date de péremption: 24 months

Détails sur FLT4

Antigène: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Autre désignation: VEGFR-3/FLT-4

Sujet: Vascular endothelial growth factor receptor-3, FLT4, PCL, LMPH1A, VEGFR3, fms-related tyrosine kinase 4,The recombinant mature sVEGFR-3/FLT-4 is a glycosylated monomeric protein. The sVEGFR-3/FLT-4 monomers have a mass of approximately 120 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis.

ID gène: 2324

NCBI Accession: NM_002020

UniProt: P35916

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling